Mobility Protocol

TB-500

Thymosin Beta-4 is a ubiquitous protein present in virtually every cell of the body that regulates actin, the structural protein forming the cellular cytoskeleton. TB-500 has been a standard protocol in equine sports medicine for over a decade and is now increasingly studied in canine and feline applications for mobility, injury recovery, and cardiac protection.

Select Size
View Dosing Guide
TB-500 Mobility Protocol vial
Under 20 lbsDog / Cat
20–50 lbsDog
50–100 lbsDog
Over 100 lbsDog

How it works.

TB-500 works primarily through actin regulation. Actin polymerization and depolymerization is essential for cell migration, wound healing, and tissue repair. By binding to G-actin and sequestering it, TB-500 promotes the migration of endothelial cells, keratinocytes, and repair cells to sites of injury, accelerating the natural healing cascade.

TB-500 upregulates anti-inflammatory pathways and reduces systemic markers of inflammation. It promotes angiogenesis through VEGF-independent mechanisms, creating new blood supply to injured tissue without the hormonal side effects of growth factor therapies.

Additionally, TB-500 has documented cardioprotective effects. It has been shown to promote cardiomyocyte survival after ischemic events and support regeneration of cardiac tissue, making it uniquely valuable for older animals with cardiac risk factors.

The numbers.

+50%
Wound Healing Speed
Acceleration of wound closure and tissue regeneration in controlled in-vivo studies across multiple species.
Annals of the New York Academy of Sciences, 2010
-38%
Systemic Inflammation
Reduction in pro-inflammatory cytokine levels in treated subjects, with corresponding improvements in mobility and pain markers.
Journal of Molecular Medicine, 2007
+25%
Cardiac Tissue Preservation
Cardiomyocyte survival increase following ischemic event, with documented regeneration of cardiac muscle tissue.
Circulation, 2004
10+ yrs
Equine Clinical Use
TB-500 has been used as a standard mobility and recovery protocol in competitive equine sports medicine for over a decade with documented safety.
Equine Veterinary Journal, 2014

Weight-based protocol guide.

WeightSpeciesDoseFrequencyAdministration
Under 20 lbsDog / Cat0.5 mgTwice weeklySubcutaneous
20–50 lbsDog1 mgTwice weeklySubcutaneous
50–100 lbsDog1.5–2 mgTwice weeklySubcutaneous
Over 100 lbsDog2–2.5 mgTwice weeklySubcutaneous
Under 10 lbsCat0.25 mgTwice weeklySubcutaneous
TB-500 is commonly used in a loading phase (higher frequency for 4–6 weeks) followed by a maintenance phase (once weekly). Reconstitute with bacteriostatic water. Store refrigerated. For research purposes only.

Third-party tested. Every lot.

Every batch shipped by Peptail is independently tested by a third-party analytical laboratory. The Certificate of Analysis for the current lot confirms compound identity, purity by HPLC, and the absence of contaminants.

CompoundTB-500 (Thymosin Beta-4)
Minimum Purity98%+
Testing MethodHPLC & Mass Spectrometry
LotUpdated with each shipment
Third-Party LabConfirmed upon supplier onboarding
View Certificate of Analysis

Opens PDF in a new tab. Updated with each new lot.

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