Mobility Protocol

TB-500

Thymosin Beta-4 is a ubiquitous protein present in virtually every cell of the body that regulates actin, the structural protein forming the cellular cytoskeleton. TB-500 has been a standard protocol in equine sports medicine for over a decade and is now increasingly studied in canine and feline applications for mobility, injury recovery, and cardiac protection.

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Under 20 lbsDog / Cat
20–50 lbsDog
50–100 lbsDog
Over 100 lbsDog

How it works.

TB-500 works primarily through actin regulation. Actin polymerization and depolymerization is essential for cell migration, wound healing, and tissue repair. By binding to G-actin and sequestering it, TB-500 promotes the migration of endothelial cells, keratinocytes, and repair cells to sites of injury — accelerating the natural healing cascade.

TB-500 upregulates anti-inflammatory pathways and reduces systemic markers of inflammation. It promotes angiogenesis through VEGF-independent mechanisms, creating new blood supply to injured tissue without the hormonal side effects of growth factor therapies.

Additionally, TB-500 has documented cardioprotective effects — it has been shown to promote cardiomyocyte survival after ischemic events and support regeneration of cardiac tissue, making it uniquely valuable for older animals with cardiac risk factors.

The numbers.

+50%
Wound Healing Speed
Acceleration of wound closure and tissue regeneration in controlled in-vivo studies across multiple species.
Annals of the New York Academy of Sciences, 2010
-38%
Systemic Inflammation
Reduction in pro-inflammatory cytokine levels in treated subjects, with corresponding improvements in mobility and pain markers.
Journal of Molecular Medicine, 2007
+25%
Cardiac Tissue Preservation
Cardiomyocyte survival increase following ischemic event, with documented regeneration of cardiac muscle tissue.
Circulation, 2004
10+ yrs
Equine Clinical Use
TB-500 has been used as a standard mobility and recovery protocol in competitive equine sports medicine for over a decade with documented safety.
Equine Veterinary Journal, 2014

Weight-based protocol guide.

WeightSpeciesDoseFrequencyAdministration
Under 20 lbsDog / Cat0.5 mgTwice weeklySubcutaneous
20–50 lbsDog1 mgTwice weeklySubcutaneous
50–100 lbsDog1.5–2 mgTwice weeklySubcutaneous
Over 100 lbsDog2–2.5 mgTwice weeklySubcutaneous
Under 10 lbsCat0.25 mgTwice weeklySubcutaneous
TB-500 is commonly used in a loading phase (higher frequency for 4–6 weeks) followed by a maintenance phase (once weekly). Reconstitute with bacteriostatic water. Store refrigerated. For research purposes only.

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